Method for freezing viable cells

Chemistry: molecular biology and microbiology – Apparatus – Microorganism preservation – storage – or transport apparatus

Reexamination Certificate

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Details

C435S002000, C435S260000, C435S298200, C435S303200, C435S809000, C062S063000, C062S380000, C062S382000

Reexamination Certificate

active

07659111

ABSTRACT:
A method and a device for improved freezing of biological containing living cells is disclosed. Directional cooling coupled with agitation, preferably rotation, of the vessel containing the biological sample allows for the freezing of large volume samples at much greater rates than before. Whether a sample is large or not, the greater thermal homogeneity during the freezing process as well as the resulting crystal morphology yield a frozen sample which is exceptionally suited for lyophilization.

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Kusakabe, H., et al., “Maintenance of genetic integrity in frozen and freeze-dried mouse spermatozoa”,Proc Natl Acad Sci U S A, vol. 98, No. 24, pp. 13501-13506, (2001).

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