Method for free radical initiated peptide sequencing

Details Method for free radical initiated peptide sequencing Method for free radical initiated peptide sequencing

2009-10-14

2011-10-11

Gakh, Yelena G (Department: 1777)

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

C546S208000

Reexamination Certificate

active

08034623

ABSTRACT:
The present invention relates to a free radical initiator and a method for peptide sequencing using the same. Compared with diazo or peroxy functionalized precursors, the precursors using the present compounds are chemically more robust and can generate radical species by homolytic cleavage upon thermal activation, enabling sequencing of a wider variety of peptides. In addition, the present invention makes it feasible to sequence peptides carrying disulfide bonds.

REFERENCES:
patent: 2007/0087447 (2007-04-01), Beauchamp et al.
Lee, M. et al. “Gas-phase peptide sequencing by TEMPO-mediated radical generation,” Analyst 2009, 134, 1706-1712; available online Jun. 12, 2009.
Hodyss, R. et al. “Bioconjugates for Tunable Peptide Fragmentation: Free Radical Initiated Peptide Sequencing (FRIPS),” J. Am. Chem. Soc. 2005, 127, 12436-12437.
Hawker, C. J. “Initiating Systems for Nitroxide-Mediated “Living” Free Radical Polymerizations: Synthesis and Evaluation,” Macromolecules 1996, 29, 5248-5254.

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