Method for fragmenting and labeling DNA involving abasic...

Details Method for fragmenting and labeling DNA involving abasic... Method for fragmenting and labeling DNA involving abasic...

2006-06-13

2006-06-13

Horlick, Kenneth R. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C436S094000

Reexamination Certificate

active

07060441

ABSTRACT:
A single- or double-stranded deoxyribonucleic acid (DNA) is fragmented and labeled by chemically fragmenting the DNA by creating at least one abasic site on the DNA, and attaching a marker to at least one of the fragments by means of a labeling reagent, the reagent covalently and predominantly coupling to at least one phosphate of the fragment. This method can be applied in the field of diagnosis.

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