Method for fractionating cells

Details Method for fractionating cells Method for fractionating cells

1980-06-02

1982-04-20

Rosen, Sam

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

435240, 435241, 424105, C12N 502, A61K 3548

Patent

active

043260268

ABSTRACT:
Enriched quantities of male and female sperm are obtained in physically separate fractions utilizing the hydrodynamic behavior of sperm in laminar flow. A flowcell fractionator is provided for performing the method, and includes a specially constructed pipette, valve and infusion pump combination.

REFERENCES:
patent: 4007087 (1977-02-01), Ericsson
patent: 4009260 (1977-02-01), Ericsson
patent: 4067965 (1978-01-01), Bhattacharya
Colligan-Parade, The San Diego Union, Sunday, Nov. 25, 1979.
Sarkar-Sperm Motility and the Effect of Aging NIH Proposal, Oct. 25, 1979, pp. 13-20.
Sarkar-Sperm Motility and X-Linked HPRT Activity etc. Abst. submitted to Am. Biophys. Soc. Jan. 9, 1980.
Ericsson et al.-Nature, vol. 246 (1973), pp. 421-424.
Roberts-Swimming and Flying in Nature, vol. 1, Plenum Press, pp. 377-393 (1974).
Roberts et al.-Nature, vol. 262 (Aug. 5, 1976), pp. 493-494.
Katz et al.-J. Theor. Biol., vol. 67 (1977), pp. 723-732.
Dmowski et al.-Fertility and Sterility, vol. 31 (1979), pp. 52-57.
Ericsson-Chem. Abst., vol. 86 (1977), p. 11860e.
Atherton-Techniques of Human Andrology (Hafez Editor), Biomed. Press (1977), pp. 173-187.
Roberts-Nature, vol. 228 (Oct. 24, 1970), pp. 375-376.
Roberts-Nature, vol. 238 (Jul. 28, 1972), pp. 223-225.

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