Method for fractional determination of aspartate aminotransferas

Details Method for fractional determination of aspartate aminotransferas Method for fractional determination of aspartate aminotransferas

1985-01-02

1988-09-06

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435184, 435193, 435810, 435886, C12Q 152, C12N 999, C12N 910, C12R 1465

Patent

active

047693231

ABSTRACT:
A method of inactivating cytosolic aspartate aminotransferase isozyme comprises addition of a specific inhibitory enzyme.
A method for the fractional determination of aspartate aminotransferase isozyme activities, which comprises (a) inactivating the cytosolic aspartate aminotransferase isozyme in a reaction mixture containing the specimen by the addition of a specific inhibitory enzyme, followed by determination of the residual mitochondrial aspartate aminotransferase isozyme activity, and (b) determination of the cytosolic isozyme activity by subtracting the activity of mitochondrial isozyme determined in (a) from the total activity of aspartate aminotransferase isozymes.
A cytosolic aspartate aminotransferase isozyme inhibiting composition contains an effective cytosolic aspartate aminotransferase isozyme inhibitory amount of a specific inhibitory enzyme.

REFERENCES:
patent: 4477566 (1984-10-01), Ricci et al.
Murao et al., Agric. Biol. Chem., 48(8):2163-2166 (Aug. 1984).
Metzler et al., Fed. Proc., 41(8):2432-2436 (1982).
Ottesen et al. and Narahashi, Methods in Enzymology, vol. 19, "Proteolytic Enzymes", Academic Press, New York, 199-215 and 651-664 (1970).
Shimizu et al., Agric. Biol. Chem., 47(8):1775-1782 (1983).
Sandmeier et al., J. Biol. Chem., 255(21):10284-10289 (1980).

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