Method for fluorometric determination of the concentrations of s

Details Method for fluorometric determination of the concentrations of s Method for fluorometric determination of the concentrations of s

1984-06-06

1986-04-01

Fields, Carolyn E.

Radiant energy

Luminophor irradiation

Methods

2504581, G01T 2164

Patent

active

045800596

ABSTRACT:
For the simultaneous measurement of the concentrations of several substances a number of fluorescence measurements corresponding to the number of substances to be tested are performed using at least one fluorescent indicator which is non-specific relative to at least one of the substances to be tested, each fluorescent indicator having different quenching constants with regard to the individual substances quenching its intensities.
From the known, unquenched fluorescence intensities of the fluorescent indicators employed, the quenched fluroescence intensities obtained by measuring, and from the quenching constants that are known, or rather, have been determined beforehand by graphical methods or calculation, the concentrations and/or concentration ratios of the individual substances are obtained.

REFERENCES:
patent: 3612866 (1971-10-01), Stevens
patent: 3725658 (1973-04-01), Stanley et al.
Hurtubise, "Selective Fluorescence Quenching and Determination of Phenolic Antioxidants", Anal. Chem. 48 (14), Dec. 1976, pp. 2092-2095.

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