Method for fluorescence spectroscopic determination of a biologi

Details Method for fluorescence spectroscopic determination of a biologi Method for fluorescence spectroscopic determination of a biologi

1983-07-14

1986-01-21

Nucker, Christine M.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Signal modification or steric inhibition

436546, 436800, 436805, 436825, G01N 33536, G01N 33533

Patent

active

045657904

ABSTRACT:
Method for fluorescence spectroscopic determination of a biological substance provided with a marker consisting of a lanthanide chelate complex formed by a lanthanide coupled to the substance via a chelate forming compound, such as an EDTA-analogue, the lanthanide ion before the detection is dissociated from the active substance for instance by adding a buffer with a pH value below 3.5 and a splitting detergent, whereafter the excitation in the determination takes place in the solution in the presence of a .beta.-diketone by using a short radiation pulse and the fluorescence from the marker is detected when the fluorescence from the noise sources has substantially ceased.

REFERENCES:
patent: 4283382 (1981-08-01), Frank et al.
patent: 4341957 (1982-07-01), Wieder
Wieder, Chem. Abs., vol. 90, 1979, 90:99557e, (citing Immounoflouresc. Tech. Proc. Int. Conf. 6th, 1978, p. 67080).
Makhijani et al., J. Ind. Chem. Soc., vol. 60, 1978, pp. 840 and 841.
Nakatani et al., Rev. Phys. Chem., Japan, vol. 42, 1972, pp. 103 to 107.
Moller et al., Chem. Rev., vol. 65, 1965, pp. 1, 10, 13 and 25 to 50.

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