Method for fixing a biological preparation

Chemistry: analytical and immunological testing – Including sample preparation

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436128, 436130, 436172, 436175, 424 75, 422 40, 435 405, 435 4052, G01N 100

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055255221

ABSTRACT:
Biological preparations or specimens are treated for a subsequent microscopic inspection. For this purpose it is necessary that the inner structure that is to be studied does not change prior to and during inspection. It is thus necessary to subject the specimens to a chemical and/or mechanical pretreatment for increasing the permeability of the specimen surface to a fixation substance in the form of an aqueous solution that contains an aldehyde fixation agent, preferably a highly volatile fixation agent or substance. The exposure of the specimens to the fixation substance takes place by immersion and/or spraying and/or fumigation. The fixation agent is preferably volatile and in the form of an aldehyde proportion of about 5% by volume or of an acrolein proportion of about 10% by volume.

REFERENCES:
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patent: 3997656 (1976-12-01), Wertlake et al.
patent: 4404181 (1983-09-01), Mauthner
patent: 4946669 (1990-08-01), Siegfried et al.
patent: 5401625 (1995-03-01), Robinson
patent: 5422277 (1995-06-01), Connelly et al.
Hayat, Fixation for Electron Microscopy, 1981, pp. 9-10, 39-41, 44-45, 58-59, 62-63, 129-130, 137-140, 314-319.
Gray, The Encyclopedia of Microscopy & Microtechnique, 1973, pp. 153-165.
Grundlagen der Histocheme (Basics of Histochemistry) by H. Luppa, Vieweg Verlag, Braunschweig 1978 section 1, pp. 42,43,52.

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