Method for fixation of biological tissue

Bleaching and dyeing; fluid treatment and chemical modification – Treatment of hides – skins – feathers and animal tissues – Internal tissues

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8 9433, 530356, 623 1, 623 2, 623 3, 623 11, 523113, A61F 202

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054475368

ABSTRACT:
A process is provided for the non-glutaraldehyde fixation of a prosthesis to be implanted in a mammal whereby the prosthesis is fixed by employing cross-linking agents to form amide linkages between and within the molecules of the prosthetic tissue. The disclosed process prevents or retards calcification and results in a product which does not cause inflammation and is nontoxic; it thus provides prostheses that are well-suited for implantation in a mammal.

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Daminik, "Structure and Properties of Crosslinked Dermal Sheep Collagen", Bioplex Medical BV, Vaals, Nederland, Chapters 7 and 8, Apr. 1993.
Harasaki et al., "Cross-Linking Agents, Degree of Cross-Linking and Calcifiability in Bioprosthetic Heart Valves", Soc. for Biomaterials, 16th Annl. Meeting, p. 25, May 20-23, 1990.
Eybl, et al., "Toxic effects of aldehydes released from fixed pericardium on bovine aortic endothelial cells," J. Biomed. Mater. Res. 23:1355-1365 (1989). No Month Available.
Nimni, et al., "Chemically modified collagen: A natural biomaterial for tissue replacement," J. Biomed. Mater. Res. 21:741-771 (1987). No Month Available.
Pereira, et al., "Effect of alternative crosslinking methods on the low strain rate viscoelastic properties of bovine pericardial bioprosthetic material," J. Biomed. Mater. Res. 24:345-361 (1990). No Month Available.

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