Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1982-09-13
1986-06-17
Shapiro, Lionel M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 70, 435 71, 4351723, 435253, 435261, 435317, 435849, 435 69, 935 29, 935 47, 935 48, 935 73, C12P 2100, C12P 2106, C12P 2102, C12P 2104, C12N 1500, C12N 120, C12N 102, C12N 100, C12R 119
Patent
active
045956588
ABSTRACT:
A method of externalizing proteins from the periplasmic space of gram-negative bacteria and in particular, E. coli and its relatives, comprising utilizing bacteria which have a phage gene, coding for a protein (such as gene III protein) or a mutant bacterial gene (such as a gene coding for a membrane function) which causes perturbation of the outer bacterial membrane resulting in leakage of proteins in the periplasmic space from the cell.
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patent: 4338397 (1982-07-01), Gilbert et al.
Webster et al.: in The Single-Stranded DNA Phages, Denhardt et al. (Ed.), Cold Spring Harbor Laboratory, 1978, pp. 557-569.
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Talmadge et al.: Gene 12, 235 (1980).
Nielsen et al.: ASM News 49(7), 318 (1983).
Lazzaroni et al.: J. Bacteriol. 145, 1351 (1981).
Holland: Nature 308, 120 (1984).
Silhavy et al.: "A Genetic Approach to the Study of Protein Localization in Escherichia coli", p. 204.
Strickberger: Genetics, 2nd Ed., MacMillan, New York, p. 577.
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Anderson et al.: J. Bacteriol. 140, 351 (1979).
Fraser: CRC Critical Reviews in Food Science and Nutrition (1982).
Beck et al.: Gene 16, 35 (1981).
Boeke Jef D.
Model Peter
Zinder Norman D.
Martinell James
Shapiro Lionel M.
The Rockefeller University
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