Method for fabricating substrate for cell culture and method for

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Solid support and method of culturing cells on said solid...

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Details

4353051, 435174, 435396, 435401, 435402, C12N 500, C12N 1100, C12M 140

Patent

active

056020295

ABSTRACT:
The substrate for cell culture to be used for cell arrangements is formed by applying a photoresist on a surface of a substrate, removing selective parts of the photoresist on the surface of the substrate by optical exposure and development, and forming an immobilized enzyme membrane on the surface of the substrate after removing the photoresist, and removing the photoresist after forming the immobilized enzyme membrane. An enzyme substrate of enzyme contained in the immobilized enzyme membrane is a material that is necessary for growth of cells for forming cell arrangements or is a material that inhibits growth of such cells, and a reaction product of oxygen contained in the immobilized enzyme membrane is a material that is necessary for growth of cells for forming cell arrangements or a material that inhibits growth of such cells. It is possible to control the cell adhesion on the surface of the substrate.

REFERENCES:
patent: 4103073 (1978-07-01), McAlear et al.
patent: 4634628 (1987-01-01), Munakata et al.
patent: 5108926 (1992-04-01), Klebe
patent: 5202227 (1993-04-01), Matsuda et al.
patent: 5470739 (1995-11-01), Akaike et al.

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