Method for extracting solutes from gel plugs

Details Method for extracting solutes from gel plugs Method for extracting solutes from gel plugs

1995-03-15

1996-06-18

Kepplinger, Esther M.

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

204462, 204613, 204616, 356344, C12Q 100, B01D 5702, B01D 6142, C25B 700

Patent

active

055276806

ABSTRACT:
A flow-through receptacle is disclosed, one end of which is designed to hold multiple plugs of gel material, containing solutes such as macromolecules, excised from electrophoretic separations, and the other end of which is capable of insertion into a recipient matrix that lies between the plates of a slab gel enclosure in an electrophoresis apparatus. The receptacle is used to place the gel plugs, containing the macromolecules or solutes, into electrophoretic contact with a recipient matrix and allows for electrophoretic transfer of the solutes from the plugs into a single concentrated zone in the recipient matrix. The concentrated macromolecules or solutes can then be further processed in the recipient gel or excised and used for procedures requiring greater amounts and concentrations of the solute than are available in the original plugs.

REFERENCES:
Fisher Catalog (1993/4) Fisher Scientific, Pgh PA p. 1193.
Biotechniques Lombard-Platet et al., vol. 15 No. 4 pp. 669-672 (1993).
Cooper T., "The Tools of Biochemistry" John Wiley & Sons, (NY) (pp. 194-233) (1977).

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