Method for extracting an insulin using enzymes which are...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S181000, C435S174000, C435S068100, C435S180000

Reexamination Certificate

active

06852513

ABSTRACT:
The invention relates to a method for catalysing complex reactions or large molecules, more specifically, to enzyme-catalysed reactions during which undesirable consecutive or subsidiary reactions usually occur; using enzymes which are bonded to a polymer support. According to the invention, undesirable consecutive or subsidiary reactions are to a large extent avoided by selecting a non-porous or almost non-porous support material. In particular, the invention relates to a method for the enzymatic extraction of biomolecules, preferably peptides, proteins, oligosaccharides or polysaccharides from their biologically inactive precursors using enzymes which are bonded to a polymer support, especially a method for extracting insulins or their analogs from the corresponding precursors using enzymes which are bonded to a polymer support. As a result of selecting a non-porous almost non-porous support material, this method leads to a selective formation of biomolecules, especially of insulins or insulin analogs and corresponding valuable substances which can be split into said insulins or their analogs, undesirable consecutive or subsidiary reactions being to a large extent avoided.

REFERENCES:
patent: 4994388 (1991-02-01), Hillegas et al.
patent: 5547929 (1996-08-01), Anderson, Jr. et al.
patent: 5641639 (1997-06-01), Perry
patent: 0294 851 (1988-12-01), None
Graessel et al., Analytical Biochemistry (1989), 180(1), 72-8.*
Mandosa, R., “Insulin Analogs: Genetic Engineering for Diabetes Control,” Diabetes Wellness Letter, Nov. 1998, pp. 1-6.
www.accessdata.fda.gov/scripts/cder/ob/docs/temptn.cfm.
Alexander Huwig et al., “Laboratory procedures for producing 2-keto-D-glucose, 2-keto-D-xylose and 5-keto-D-fructose from D-glucose, D-xylose and L-sorbose with immobilized pyranose oxidase ofPeniophora gigantea,” Journal of Biotechnology32(3):309-315 (1994).
P. Chr. Lorenzen et al., “Characterization of trypsin immobilized on ixirane-acrylic beads for obtaining phosphopeptides from casein,”Z. Ernährungswiss34(2):118-130 (1995).
H. Eckstein et al., “Immobilization of Papain and Trypsin on Epoxy-Polymers for Enzyme-Catalyzed Peptide Synthesis,”Chemistry of Peptides and Proteinsvols. 5/6(A):211-216 (1993).
Derwent Abstract of EP 0294 851 A2.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for extracting an insulin using enzymes which are... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for extracting an insulin using enzymes which are..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for extracting an insulin using enzymes which are... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3472241

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.