Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2003-06-19
2009-02-17
Minnifield, N. M (Department: 1645)
Chemistry: molecular biology and microbiology
Vector, per se
C435S069100, C435S069700, C435S071100, C435S071200, C435S091400, C435S252300, C435S252330, C435S471000, C435S476000
Reexamination Certificate
active
07491528
ABSTRACT:
The present invention provides an expression vector comprising genes encoding OmpF ofE. coliand a desired protein,E.colitransformed with the expression vector, and a method for extracellular production of desired proteins by employing the same. The recombinant expression vector of the invention comprises an ampicillin-resistance gene, the OmpF promoter and the OmpF gene. In accordance with the invention, a desired protein can be produced extracellularly by a simpler method than conventional methods such that: secretory production of OmpF fusion protein begins simultaneously with growth of the cells through constitutive expression employing an OmpF promoter, and as the concentration of cells increases, the amount of secretory production of the protein also increases continuously. Therefore, desired proteins can be produced in large quantities by a high concentration culture of cells.
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Jeong Ki-Jun
Lee Sang-Yup
Knobbe Martens Olson & Bear LLP
Korea Advanced Institute of Science and Technology
Minnifield N. M
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