Method for expression of small RNA molecules within a cell

Details Method for expression of small RNA molecules within a cell Method for expression of small RNA molecules within a cell

2002-09-13

2010-06-08

Zara, Jane (Department: 1635)

Chemistry: molecular biology and microbiology

Vector, per se

C435S006120, C435S091100, C435S091310, C435S455000, C536S023100, C536S024300, C536S024500

Reexamination Certificate

active

07732193

ABSTRACT:
The invention provides methods and compositions for the expression of small RNA molecules within a cell using a lentiviral vector. The methods can be used to express doubles stranded RNA complexes. Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell, which are capable of down regulating the expression of a target gene through RNA interference. A variety of cells can be treated according to the methods of the invention including embryos, embryogenic stem cells, allowing for the generation of transgenic animals or animals constituted partly by the transduced cells that have a specific gene or a group of genes down regulated.

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