Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1988-06-17
1991-07-16
Martinell, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
43525231, 4353201, 435 694, 435 695, 435 692, 435 693, 435 6951, 435 6952, 435 696, 435 697, 435 698, 435 699, C12P 2100, C12P 2102, C12N 1574, C12N 121
Patent
active
050325108
ABSTRACT:
The present invention discloses a novel method for expressing and secreting a functional polypeptide in Bacillus including the vectors and transformants for the practice thereof. The vectors comprise the transcriptional and translational activity sequence, and optionally the signal peptide coding sequence, of the Staphylococcus aureus nuclease gene. The vectors and transformants are useful for the production and secretion of human hormones such as human proinsulin.
REFERENCES:
patent: 4783405 (1988-11-01), Kovacevic et al.
Lovett et al., "Bacillus subtilis as a Host for Molecular Cloning", Methods in Enzymology 68: 342 (1979).
Webster's New Collegiate Dictionary, G. & C. Merriam Company, (1979), Springfield, Mass., p. 461.
Shortle, 1983, Gene 22:181.
Palva, 1982, Gene 19:81.
Palva et al., 1982 Proceedings National Academy of Science, U.S.A. 79:5582.
Derwent Abstract 37323 E/19 (1982).
Saunders et al., 1984, J. Bacteriology 157:718.
Fairweather et al., 1983, Infectious Immunology 41:1112.
U.S. patent application Ser. No. 459,792.
Palva et al., 1983, Gene 22:229.
Kovacevic Steven
Miller James R.
Eli Lilly and Company
Jones Joseph A.
Martinell James
Whitaker Leroy
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