Method for expression and isolation of biologically active molec

Chemistry: molecular biology and microbiology – Vector – per se

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4351723, 435 691, 436 241, 935 23, 935 34, C07H 2104, C12N 1509, C12N 1563, C12N 1500

Patent

active

058245430

ABSTRACT:
A recombinant nucleic acid construct is presented in which a mouse uroplakin-II (UP-II) gene promoter is coupled to a heterologous gene of interest. When the resultant construct is introduced into a transgenic mouse, the UP-II promoter directs the expression of the heterologous gene in the urothelium of the said mouse.

REFERENCES:
Garver et al., Gene Therapy, 1(1):46-50, 1994.
Meyer-Puttlitz et al., Neuro Report, 6:1674-1678, 1995.
Lin et al., PNAS USA, 92:679-683, 1995.
Frohman et al., "Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer", Proc. Nat'l Acad. Sci. USA 1988, 85, 8998-9002.
Hicks, R.M., "The Fine Structure of the Transitional Epithelium of Rat Ureter", J. Cell Biol. 1965, 26, 25-48.
Hicks, R.M., "The Mammalian Urinary Bladder: An Accommodating Organ", Biol. Rev. 1975, 50, 215-246.
Koss, L.G., "The Asymmetric Unit Membranes of the Epithelium of the Urinary Bladder of the Rat", Lab. Invest. 1969, 21, 154-168.
Lin et al., "Precursor Sequence Processing, and Urothelium-specific Expression of a Major 15-kDa Protein Subunit of Asymmetric Unit Membrane", J. Biol. Chem. 1994, 269, 1775-1784.
Mercer et al., "The Dopamine .beta.-Hydroxylase Gene Promoter Directs Expression of E. coli lacZ to Sympathetic and Other Neurons in Adult Transgenic Mice", Neuron 1991, 7, 703-716.
Peschon et al., "Spermatid-specific expression of protamine 1 in transgenic mice", Proc. Natl. Acad. Sci. USA 1987, 84, 5316-5319.
Ryan et al., "Chromosomal localization of uroplakin genes of cattle and mice", Mamm. Genome 1993, 4, 656-661.
Staehelin, L.A., "Lumenal Plamsa Membrane of the Urinary Bladder", J. Cell Biol. 1972, 53, 73-91.
Wu, X.-R. and Sun, T.-T., "Molecular cloning of a 47 kDa tissue-specific and differentiation-dependent urothelial cell surface glycoprotein", J. Cell Sci. 1993, 106, 31-43.
Wu et al., "Large Scale Purification and Immunolocalization of Bovine Uroplakins I, II, and III", J. Biol. Chem. 1990, 265, 19170-19179.
Wu et al., "Mammalian Uroplakins", J. Biol. Chem. 1990, 265, 19170-19179.
Wu et al., "Uroplakins Ia and Ib, Two Major Differentiation Products of Bladder Epithelium, Belong to a Family of Four Transmembrane Domain (4TM) Proteins", J. Biol. Chem. 1994, 269, 13716-13724.
Yu, J., "Uroplakin I: A 27-kD Protein Associated with the Asymmetric Unit Membrane of Mammalian Urothelium", J. Cell Biol. 1994, 125, 171-182.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for expression and isolation of biologically active molec does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for expression and isolation of biologically active molec, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for expression and isolation of biologically active molec will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-243533

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.