Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-11-06
1998-09-15
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4352523, 43525233, 4353201, 530350, 530360, 530832, 536 232, 536 235, 424535, C12P 2106, C12N 120, C07K 100, C07H 2104
Patent
active
058077022
ABSTRACT:
The present invention provides a method of producing phophorylated, recombinant human .beta.-casein having 3 to 5 phosphates in a bacterial cell using a single vector containing in tandem both the nucleotide sequence encoding .beta.-casein and a sequence encoding the enzyme CKII .beta..alpha. which is capable of phosphorylating human .beta.-casein.
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Biochemical and Biophysical Research Communications, vol. 203, No. 1, Aug. 30, 1994, pp. 225-230, Jeng et al., "Production of an Enzymatically Active E1 Component of Human Pyruvate Dehydrogenase Complex in Eschericia coli: Support Role of E1 Beta Subunit in E1 Activity".
Proceedings of the National Academy of Sciences, USA, vol. 91, Mar. 15, 1994, pp. 2767-2771, Shi et al., "Expression of Recombinant Human Cesein Kinase II and Recombinant Heat Shock Protein 90 in Escherichia coli and Characterization of Their Interactions".
Protein Expression and Purification, vol. 4, No. 5, Oct. 1993, pp. 373-381, Hansson, et al., Expression of Human Milk .beta.-Casein in Eschericia coli: Comparison of Recombinant Protein with Native Isoforms.
Journal of Biological Chemistry, vol. 259, No. 8, (1993), pp. 5132-5138, Greenberg, et al., "Human .beta.-Casein, Amino Acid Sequence and Identification for Phosphorylation Sites".
Baxter Jeffrey Harris
Hansson Lennart
Hards Robert George
Mukerji Pradip
Thurmond Jennifer Marie
Abbott Laboratories
Becker Cheryl L.
Saidha Tekchand
Wax Robert A.
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