Multicellular living organisms and unmodified parts thereof and – Method of using a transgenic nonhuman animal to manufacture...
Reexamination Certificate
2006-08-08
2006-08-08
Chen, Shin-Lin (Department: 1632)
Multicellular living organisms and unmodified parts thereof and
Method of using a transgenic nonhuman animal to manufacture...
C800S013000, C800S017000, C800S021000, C800S022000, C800S025000, C435S320100, C536S023500
Reexamination Certificate
active
07087808
ABSTRACT:
The present invention discloses a method for expressing multiple recombinant proteins in transgenic non-human mammalian milk, characterized in which human clotting factor IX gene and porcine lactoferrin gene are transferred into the mammal by gene injection and embryonic implantation to obtain expression in the milk of transgenic mammal and its filial generation. The method of this invention can maintain the stable expression of multiple recombinant proteins in the transgenic mammal during lactation and stable expression amount proximate to that of the first generation in the offsprings of the transgenic non-human mammal.
REFERENCES:
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Mercier et al. “The modification of milk protein composition through transgenesis: progress and problems,” In: Transgenic Animals: Generation and use, Ed. Houdebine LM, Harwood Academic Publishers, The Netherlands pp. 473-482, 1997.
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Dyck et al. “Making recombinant proteins in animals—different systems, different applications.” Trends in Biotechnology 2(9):394-399, 2003.
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Sigmund, C., Jun. 2000, Arterioscler. Thromb. Vasc. Biol., p. 1425-1429.
Chen Chuan Mu
Cheng Teng Kuei
Lin Shau Ping
Wu Shinn Chih
Yang Ping Cheng
Animal Technology Institute Taiwan
Bacon & Thomas PLLC
Chen Shin-Lin
LandOfFree
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