Method for expressing and purifying proteins using budded...

Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Recovery or purification

Reexamination Certificate

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C435S456000, C435S069700

Reexamination Certificate

active

07070978

ABSTRACT:
Method for efficiently expressing a protein in an active form by using baculovirus expression system wherein the protein is selected from a membrane-bound enzyme, a substrate of the membrane-bound enzyme, a membrane-bound enzyme activator, a membrane-bound transport protein, a channel protein, a membrane structural protein, a protein involved in adhesion, a protein involved in antigen presentation, or a protein involved in formation of high dimensional structure of a protein. There is provided a method for expressing such a protein by culturing a host infected with at least one type of recombinant baculovirus which contains a gene encoding the protein, wherein the protein is expressed in a budded baculovirus released from the host.

REFERENCES:
patent: 5858750 (1999-01-01), Bandman et al.
patent: 2002/0052040 (2002-05-01), Hunt
patent: 98/46777 (1998-10-01), None
patent: 01/02551 (2001-01-01), None
Ikeda et al. PNAS. 1995; 92: 126-130.
Loisel et al. Nature Biotechnology. Nov. 1997; 15: 1300-1304.
Grabherr et al. Biotechniques. 1997; 22 (4): 730-735.
Boublik et al. Biotechnology. Oct. 1995; 13: 1079-1084.
Possee. Current Opinion in Biotechnology. 1997; 8: 569-572.
Nohturefft et al. PNAS. 1999; 96: 11235-11240.
Duncan et al. Journal of Biological Chemistry. 1997; 272 (19): 12778-12785.
Fields, Virology, Third Edition, vol. 1, p. 539.
Lanford et al., “Expression and Characterization of Hepatitis B Virus Surface Antigen Polypeptides in Insect Cells with a Baculavirus Expression System,” Journal of Virology, vol. 63, No. 4; pp. 1549-1557 (1989).

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