Method for expansion of epithelial stem cells

Prosthesis (i.e. – artificial body members) – parts thereof – or ai – Implantable prosthesis – Having means to promote cellular attachment

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C623S005160, C623S915000

Reexamination Certificate

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10240310

ABSTRACT:
Transplantation of epithelial stem cells, cultured ex vivo on specifically treated amniotic membrane, yields, with that amniotic membrane, a surgical graft having expanded epithelial stem cells. The method of creating this graft and the graft itself are simple and effective to reconstruct damaged tissue, a preferred example being corneal tissue. The source of the epithelial stem cells can be a very small explant from healthy autologous and allogeneic tissue biopsy. The amniotic membrane is treated such that its extracellular matrix is maintained, but its cells are killed.

REFERENCES:
patent: 2002/0039788 (2002-04-01), Isseroff et al.
patent: 2001-161353 (2001-06-01), None
patent: WO 98/37903 (1998-09-01), None
U.S. Appl. No. 60/185,744, filed on Feb. 29, 2000.
Wikipedia topic entitled “Stem cell”, five pages, from http://en.wikipedia.org/wiki/Stem—Cell, accessed on Sep. 17, 2006.
Schwab, “Composite amniotic membrane and corneal epithelial cell”, Annu. meeting of the assoc. for research in Vis. & Ophth, May 14, 1999, vol. 40, No. 4, pp. s578.
Schwab, “Cultured coreneal epithelial for ocular surface disease”, Transactions of the American Ophthalmological Society, 1999, vol. 97, pp. 891-986.
Tsai, “Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells”, The New Eng. J. of Med., Jul. 13, 2000, Vol. 343, No. 2., pp. 86-93.
Grueterich, “Ex vivo expansion of limbal epithelial stem cells: amniotic membrane serving as a stem cell niche”, Survey of Ophth., 2003, vol. 48, No. 6., pp. 631-646.
Tseng, “Amniotic Membrane Transplantation for Conjunctival Surface Reconstruction,” Am. J. of Ophthalmology,vol. 124(6), pp. 765-774 (1997).

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