Method for excystation of sporocysts

Drug – bio-affecting and body treating compositions – Inorganic active ingredient containing – Elemental chlorine or elemental chlorine releasing inorganic...

Reexamination Certificate

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C435S032000

Reexamination Certificate

active

11206372

ABSTRACT:
A process to excystate protozoal sporocysts involves treatment of an infected tissue sample with a sodium hypochlorite solution to stimulate excystation of the sporocysts from the tissue. Thereafter, removal of the sodium hypochlorite solution and treatment of the sample with a cell culture media as the excystation fluid eliminates incubation and subsequent washing steps using expensive reagents. The excystation fluid contains substantially no chelating agents, proteins, enzymes or bile acids.

REFERENCES:
patent: 6344340 (2002-02-01), Dibner et al.
patent: WO 01/15708 (2001-03-01), None
Dubey, J.P., et al., “Sarcocystosis of Animals and Man,” 1989, p. 98-99, CRC Press, Inc., Boca Raton, Florida.
Murphy, A.J., et al., “Simplified Technique for Isolation, Excystation, and Culture of Sarcocystis Species from Opossums,” J. Parasitol., 1999, p. 979-981, vol. 85(5).
Cawthorn, R.J. et al., “In Vitro Excystation of Sarcocystis Capracanis Sarcocystis Cruzi and Sarcocystis Tenella,” J. Parasitol., 1986, p. 880-884, vol. 72(6).
McKenna, P. B., et al., “The Survival ofSarcocystis GiganteaSporocysts Following Exposure to Various Chemical and Physical Agents”, Veterinary Parasitology, 1992 pp. 1-16, vol. 42.

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