Method for excystation of sporocysts

Details Method for excystation of sporocysts Method for excystation of sporocysts

2005-09-27

2005-09-27

Naff, David M. (Department: 1651)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Protozoa, media therefor

C435S029000, C435S242000, C435S325000

Reexamination Certificate

active

06949375

ABSTRACT:
A process to excystate protozoal sporocysts involves treatment of an infected tissue sample with a sodium hypochlorite solution to stimulate excystation of the sporocysts from the tissue. Thereafter, removal of the sodium hypochlorite solution and treatment of the sample with a cell culture media as the excystation fluid eliminates incubation and subsequent washing steps using expensive reagents. The excystation fluid contains substantially no chelating agents, proteins, enzymes or bile acids.

REFERENCES:
patent: WO 01/15708 (2001-03-01), None
McKenna, P.B. et al., 1992. Veterinary Parasitology, vol. 42, pp. 1-16.
Dubey, J.P., et al., “Sarcocystosis of Animals and Man,” 1989, p. 98-99, CRC Press, Inc., Boca Raton, Florida.
Murphy, A.J., et al., “Simplified Technique for Isolation, Excystation, and Culture ofSarcocystisSpecies from Opossums,” J. Parasitol., 1999, p. 979-981, vol. 85(5).
Cawthorn, R.J. et al., “In Vitro Excystation ofSarcocystis Capracanis Sarcocystis CruziandSarcocystis Tenella,” J. Parasitol., 1986, p. 880-884, vol. 72(6).

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