Method for evaluation of the ratio of amounts of biomolecules or

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification

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435 2, 435 71, 435 74, 435262, 435 41, 435810, 435813, 4352891, 4352831, 435304, 436 66, 436 63, 436 8, 436 15, 436174, 436177, 436536, 436538, 422 61, 210634, 210635, 210639, 548104, 548108, 548403, G01N 3353, G01N 2100, G01N 3100, C07C 102, G07D23100

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active

061369608

ABSTRACT:
The present invention is directed to a method of determining the ratio of the concentration or amounts of two or more chemical compounds and/or biopolymers, or of the relative amounts of sub-populations of closely structurally related compounds, in a multi-component mixture, extract, system, and the like using an analytical physicochemical process. The method is based on distribution of the components of the mixture (system, extract, etc.) between two or more immiscible phases and the subsequent determination of the total amounts (concentrations) of biopolymers (chemical compounds) or of the amounts (concentrations) of one or more component(s) or sub-populations of the system in the phases. The ratio between these concentrations is defined therein as the partition coefficient. The partition coefficient is used as a measure of the ratio of the amounts of two or more biopolymers (chemical compounds) or their sub-populations in a multi-component mixture (extract, system, etc.), when calibrated against a series of sample systems of the same type with varied ratios of components.

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