Chemistry: analytical and immunological testing – Rate of reaction determination
Reexamination Certificate
1998-02-26
2001-06-12
Soderquist, Arlen (Department: 1743)
Chemistry: analytical and immunological testing
Rate of reaction determination
C422S073000, C422S082050, C436S055000, C436S069000, C436S070000
Reexamination Certificate
active
06245569
ABSTRACT:
The invention relates to a method for the accurate evaluation of reaction kinetics, which are evaluated using the so-called threshold-value method. Curve profiles of this type occur whenever, under controlled ambient conditions, the transition of a reaction-dependent variable from an initial state to a final state is observed over time.
The threshold-value method is a method which is frequently used in practice. A method of this type is, for example, described by KOLDE (Behring Inst. Mitt. 78, p. 176-187 (1985)).
A way of characterizing an object to be measured on the basis of a specific measuring system using a 2-step method has already been described. In this case
a) by adding certain activation components in the object to be measured, a process is initiated which delivers a signal over time on the basis of a defined detection principle. This gives a series of measurements (T
i
,S
i
), i=1, . . ,n. In this case, T
i
denotes the time of the i-th instant and S
i
denotes the signal at time T
i
.
b) The series of measurements (T
i
,S
i
) i=1, . . . ,n is reduced with the aid of a transformation f to a raw value R:
(
T
i
,
S
i
)
,
i
=
1
,
…
,
n
→
f
R
The threshold-value method is such a transformation f as is needed in step b). It is essentially characterized by a parameter d which establishes the actual threshold to be used. The raw value is in this case defined as the time at which, starting from a base value, the series of measurements persistently exceeds the threshold for the first time.
The known method cannot correctly evaluate curve profiles which have an inconstant profile, as represented in FIG.
1
. In the example given in this figure, the correct raw value would have been about 38 s (persistent exceeding of a predetermined threshold), but was actually determined at about 25 s using the known method. Depending on the field of application, a miscalculation of this type may have serious consequences and even, in the extreme, lead to life-threatening situations (see examples).
The object of the present invention was therefore to provide a method which eliminates the abovementioned disadvantages. This means that miscalculations, as may arise when using the described method, can be made as improbable as is technically feasible.
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Dade Behring Marburg GmbH
Finnegan, Henderson Farabow, Garrett and Dunner L.L.P.
Soderquist Arlen
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