Method for evaluating immunogenicity

Details Method for evaluating immunogenicity Method for evaluating immunogenicity

1990-03-15

1992-08-04

Kepplinger, Esther L.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436547, 436815, 436821, 436824, 424 858, 530413, 935 76, G01N 33543

Patent

active

051358743

ABSTRACT:
A method for evaluating the potential immunogenicity of a protein derived from recombinant DNA technology. The method involves injecting an animal with the recombinant protein and then isolating antiserum from the animal. The antiserum is depleted of antibodies to a reference protein, i.e., a plasma derived protein, by adsorbing the antiserum against the reference protein. The adsorbed antiserum is then blotted against the recombinant protein, to see if any antibodies were produced which recognize the recombinant protein, but did not recognize the plasma-derived protein during adsorption.

REFERENCES:
Courtney et al., "High-level production of biologically active human .alpha., antitrypsin in Escherichia coli", Proc. Natl. Acad. Sci. USA 81:669-673 (Feb. 1984).
Courtney et al., "Synthesis in E. coli of .alpha., -antitrypsin variants of therapeutic potential for emphysema and thrombosis," Nature 313:149-151 (10 Jan. 1985).

Affiliated with

Also associated with

Rating

Method for evaluating immunogenicity does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for evaluating immunogenicity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for evaluating immunogenicity will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-777947