Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1988-06-01
1992-09-15
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
436518, 436530, 436532, 436547, 436824, 424 9, 424 858, 530413, C12Q 168, G01N 33543
Patent
active
051477793
ABSTRACT:
A method for evaluating the potential immunogenicity of a protein derived from recombinant DNA technology. The method involves injecting an animal with the recombinant protein and then isolating antiserum from the animal. The antiserum is depleted of antibodies to a reference protein, i.e., a plasma derived protein, by adsorbing the antiserum against the reference protein. The adsorbed antiserum is then blotted against the recombinant protein, to see if any antibodies were produced which recognize the recombinant protein, but did not recognize the plasma-derived protein during adsorption.
REFERENCES:
patent: 4746647 (1988-05-01), Svenson
patent: 4769320 (1988-09-01), Furie et al.
Ritchie "Preparation of Polyclonal Antisera"Chapter 2, Manual of Clinical Laboratory Immunology 1986 pp. 4-8.
Voller et al. "Enzyme-Linked Immunosorbent Assay" Chapter 17, Manual of Clinical Laboratory Immunology 1986 pp. 99, 103-104.
Smith et al. "Monoclonal Antibody Screening: Two Methods Using Antigens Immobilized on Nitrocellulose" Analytical Biochemistry 138 (1984) pp. 119-124.
Esmon P. C., Mitra I., Fournel M. A. 1988; Recombinant Factor VIII Immunogenicity studies. FASEBJ 2(5)5027.
Esmon Pamela C.
Fournel Michael A.
Aston David
Bidwell Carol E.
Enayati Elizabeth F.
Giblin James A.
Kepplinger Esther L.
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