Method for evaluating drug metabolism and reagent compositions t

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 4, 435 29, 435176, 435177, 435968, 435963, 435370, 435366, 424 92, C12Q 126, C12Q 100, C12Q 102

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059898445

ABSTRACT:
This invention relates to a method for evaluating the susceptibility of pharmaceutical drugs to metabolism by a specific cytochrome P-450 isozyme, which comprises contacting the sample compound with a reagent composition prepared by adding the specific cytochrome P-450 isozyme to liver microsomes lacking said specific cytochrome P-450 isozyme in a carrier material. More particularly, this invention relates to a useful in vitro quantitative assay for drug metabolism by P-450 isozymes such as CYP2D6, CYP2C19, and CYP2A6. This invention also relates to a reagent composition which is useful for said in vitro assay.

REFERENCES:
patent: 5240831 (1993-08-01), Barnes
patent: 5478723 (1995-12-01), Parkinson et al.
patent: 5525482 (1996-06-01), States et al.

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