Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1981-12-16
1988-05-10
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436533, 436534, 436824, 436500, G01N 33535, G01N 33546
Patent
active
047435364
ABSTRACT:
Method for the determination of a component of the reaction between a specific binding protein and a substance bindable therewith which process comprises incubating a sample solution with a determined amount of binding component in insoluble form in the presence of a reagent containing a known amount of one of the binding components in enzyme-labeled form, separating the liquid phase from the solid phase by centrifuging and determining the activity of the enzyme remaining in the liquid phase in a centrifuge rotor during or after the action of increased gravitional force whereby the solid phase and the liquid phase are still in contact with one another.
The present invention provides a process for the determination of a component of the reaction between a specific binding protein and a substance bindable therewith by incubation of a sample solution with a previously ascertained amount of binding component in insoluble form in the presence of a reagent which contains a known amount of one of the binding components in enyzme labeled form, separation of the liquid phase from the solid phase by centrifuging and determination of the activity of the marker enzyme remaining in the liquid phase, wherein measurement of the enzyme activity is carried out in a centrifuge rotor during or after the action of increased gravitational force whereby the solid phase and the liquid phase are still in contact with one another.
REFERENCES:
patent: 3654090 (1972-04-01), Schuurs et al.
patent: 4299916 (1981-11-01), Litman et al.
Honig et al., Editor, The Van Nostrand Chemist's Dictionary, D. Van Nostrand Co., Inc., Princeton, N.J., p. 675 (1953).
Grant, Ed., Hackh's Chemical Dictionary, Fourth Edition, McGraw-Hill Book Company, N.Y., p. 649 (1969).
Albert Winfried
Evanega George R.
Boehringer Mannheim GmbH
Kepplinger Esther M.
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