Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1983-09-29
1987-04-07
Schain, Howard E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435105, 435190, 435194, C12P 1900, C12P 1902, C12N 904, C12N 912
Patent
active
046561331
ABSTRACT:
Utilizing the method of the present invention, a variety of aldose and ketose phosphates may be synthesized, labeled with .sup.13 C at any one of a number of single sites or synthetically related sites starting from .sup.13 C labeled pyruvate and using enzymes of the glycolytic pathway. The method of the present invention provides a quick and convenient method for the introduction of the .sup.13 C isotope into a variety of carbohydrates in a single step reaction. It also provides a method for the preparation of isotopically labeled carbohydrates in high yield, with little or no limitation on quantity, from commercially available labeled precursor.
REFERENCES:
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patent: 4440854 (1984-04-01), Whitesides et al.
Serianni, A. S. et al., "Carbon-13-Enriched Carbohydrates: Preparation of Triose, Tetrose and Pentose Phosphates", 18 Biochemistry 1192-1199 (1979).
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Serianni, A. S. et al., "Carbon-13-Enriched Carbohydrates, Preparation of Erythrose, Threose, Glyceraldehyde and Glycoaldehyde with .sup.13 C-Enrichment in Various Carbon Atoms", 72 Carbohydrate Res. 79-91 (1979).
Rognstad, R. et al., "Enzymic Synthesis of Glucose-4-Tritium and Glucose-3-Tritium", 109 Archives of Biochemistry and Biophysics 372-375 (1965).
Hauska, Von Gunther et al., "Enzymatische Synthese Von [5-.sup.3 H] D-Fructose-1, 6-Biphosphat, [5-.sup.3 H] D-Glucose-6-Phosphat und [5-.sup.3 H] D-Glucose", 348 Hoppe-Seyler's Z. Physiol. Chem. 1273-1276 (1967).
Cohn, M. et al., "Nuclear Magnetic Resonance Assignment of the Vinyl Hydrogens of Phosphoenolpyruvate, Stereochemistry of the Enolase Reaction", 92 J. Am. Chem. Soc. 4095-4098 (1970).
Colowick, S. P. et al., (Eds), Methods in Enzymology, New York, Academic Press, 1955-Present, See: Serianni, A. S. et al., vol. 89 (1982), pp. 83-92, Mandl, I. et al., vol. 3 (1957), pp. 165-167, Korenberg, A., vol. 1 (1955), pp. 441-443.
Boyer, P. D. (Ed.), The Enzymes, 3rd Ed. New York, Academic Press, 1973, See: Nada, L., vol. 8, p. 302. Watts, D. C. vol. 8, pp. 428-429.
Board of Regents University of Texas System
Schain Howard E.
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