Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Chemical stimulation of growth or activity by addition of...
Patent
1990-10-05
1992-04-21
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Chemical stimulation of growth or activity by addition of...
435243, 435 692, 435 3, C12N 138
Patent
active
051067471
ABSTRACT:
A multienzyme system capable of reducing waste products and synthesizing nutrients in a cell culture system is disclosed. In one embodiment, lactic dehydrogenase and leucine dehydrogenase are employed in conjunction with amino acid precursors and a recycling co-factor to achieve the synthesis of amino acids and a reduction in the levels of ammonia and lactic acid. The multienzyme system of the invention may be added directly to the cell culture system or it may be microencapsulated. Alternatively, the invention may be utilized in conjunction with a container unit in which cell culture medium contacts the multienzyme system external to the main culture vessel compartment.
REFERENCES:
patent: 4891310 (1990-01-01), Shimizu et al.
Wandrey Chem. Abstracts, vol. 98, 1983, Abstract #98: 51907g.
Gu. Biomat., Art. Cells & Art Org. 15(1), 297-303 (1987).
Butler, M., Develop. Biol. Standard. 60:269-280 (1985).
Chang et al., Trans. Am. Soc. Artif. Intern. Organs XXIV:18-20 (1978).
Gu et al., Biomat., Art. Cells, Art. Org., 15(1):297-303 (1987).
Chang and Malouf, Trans. Am. Soc. Artif. Intern. Organs 24:18-20 (1978).
Gu and Chang, Applied Biochemistry and Biotechnology, Weetall (Ed.), pp. 115-124, The Humana Press Inc. (1990).
Gu and Chang, J. Mol. Catalysis 62:331-339 (1990).
Gu and Chang, Biotechnol. and Bioengineering 36:263-269 (1990).
Gu and Chang, Biotech. Appl. Biochem. 12:227-236 (1990).
Life Technologies Inc.
Robinson Douglas W.
Williams Jane A.
LandOfFree
Method for enzymatic regeneration of cell culture media and medi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for enzymatic regeneration of cell culture media and medi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for enzymatic regeneration of cell culture media and medi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1585551