Method for enumeration of oral gram-negative bacteria

Details Method for enumeration of oral gram-negative bacteria Method for enumeration of oral gram-negative bacteria

1981-10-01

1983-04-05

Fagelson, Anna P.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

2504612, 424 87, 250305, 424 92, 435 4, 435 7, 435 34, 435822, 436546, 436800, 436805, 436825, G01N 2164, G01N 3354, C12Q 104, C12Q 124

Patent

active

043791352

ABSTRACT:
A reagent for the enumeration of oral gram-negative bacteria, such as Bacteriodes melaninogenicus, Capnocytophaga sp. and Eikenelia corrodens, by an indirect fluorescent antibody method is provided which utilizes an unlabelled antiserum to oral gram-negative bacteria and a fluorescent conjugated antibody obtained by immunizing an animal of a different species than the antiserum with .gamma.-globulin of an animal of the same species as the antiserum. A sample containing oral gram-negative bacteria to be enumerated is treated with the antiserum and the reaction product obtained is then treated with the fluorescent conjugated antibody to enumerate the bacteria in the sample by observing under a fluorescent microscope.

REFERENCES:
patent: 3931398 (1976-01-01), Gaffar
Nairn, Fluorescent Protein Tracing, Churchill Livingstone, NY 4th Ed., 1976, pp. 163-167, 170, 171, 175-180, 185, 188, 189, 192, 193.
Stauffer, J. Clin. Microbiol. vol. 2, Oct. 1975, pp. 337-344.
Jones, Diss. Abs., vol. 35, No. 8B, p. 4018B.
Humphries, Dissertation, University Microfilms, Ann Arbor, MI, No. 71-8271, Baylor U., 1970, pp. 39-44, 104, 105.
Takeuchi, Shika Igaku, J. Osaka Odontol. Soc., vol. 33, No. 3, 1970, pp. 386-404.
Shuji Sasaki, "Biological Activity of Lipopolysaccharides Isolated from Bacteria in Human Periodontal Lesions", Nov., 1979; The Bulletin of Tokyo Dental College, vol. 20 (No. 4), pp. 159-174.
J. T. Irving et al., "Histological Changes in Experimental Periodontal Disease in Rats Monoinfected with Gram-Negative Organisms", J. Periodontal Res. 13 (Jul.): 326-332, 1978.
S. S. Socransky, "Microbiology of Periodontal Disease-Present Status and Future Considerations", J. Periodontol., vol. 48, No. 9, Sep., 1977, pp. 497-504.
J. T. Irving, International Association for Dental Research, Abstract No. 783, 1976.
S. Sasaki et al., American Association for Dental Research, Abstract No. 422, 1977.
J. T. Irving, American Association for Dental Research, Abstract No. 423, 1977.
M. G. Newman et al., "Studies of the Microbiology of Periodontosis", J. Periodontol. Jul., 1976, pp. 373-379.

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