Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample
Reexamination Certificate
2011-01-11
2011-01-11
Gabel, Gailene R (Department: 1641)
Chemical apparatus and process disinfecting, deodorizing, preser
Analyzer, structured indicator, or manipulative laboratory...
Means for analyzing liquid or solid sample
C422S067000, C422S073000, C422S082080, C422S082090, C435S006120, C435S007200, C435S007250, C435S287200, C435S973000, C436S521000, C436S063000, C436S172000
Reexamination Certificate
active
07867447
ABSTRACT:
A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample. In particular, the use of the second antibody prevents interference by platelet-associated aggregates in the scoring procedures.
REFERENCES:
patent: 4325706 (1982-04-01), Gershman et al.
patent: 4727020 (1988-02-01), Recktenwald
patent: 5858667 (1999-01-01), Dertinger et al.
patent: 6100038 (2000-08-01), Dertinger et al.
patent: 6287791 (2001-09-01), Terstappen et al.
patent: 2003/0134305 (2003-07-01), Dertinger et al.
patent: 2007/0274919 (2007-11-01), Dertinger
patent: 0869347 (1998-10-01), None
patent: 03016866 (2003-02-01), None
Torous et al., “Enumeration of Micronucleated Reticulocytes in Rat Peripheral Blood: A Flow Cytometric Study,” Mutat. Res. 465:91-99 (2000).
Torous et al., “Flow Cytometric Enumeration of Micronucleated Reticulocytes: High Transferability Among 14 Laboratories,” Environ. Mol. Mutagen. 38:59-68 (2001).
Torous et al., “Interlaboratory Validation of a CD71-Based Flow Cytometric Method (MicroFlow®) for the Scoring of Micronucleated Reticulosytes in Mouse Peripheral Blood,” Environ. Mol. Mutagen. 45:44-55 (2005).
Wakata et al., “Evaluation of the Rat Micronucleus Test with Bone Marrow and Peripheral Blood: Summary of the 9th Collaborative study by CSGMT/JEMS MMS,” Environ. Mol. Mutagen. 32:84-100 (1998).
Widel et al., “Micronucleus Assay in vivo Provides Significant Prognostic Information in Human Cervical Carcinoma; The Updated Analysis,” Int. J. Radiat. Biol. 77(5):631-636 (2001).
Widel et al., “The Increment of Micronucleus Frequency in Cervical Carcinoma During Irradiation in Vivo and Its Prognostic Value for Tumor Radiocurability,” Br. J. Cancer 80:1599-1607 (1999).
Zolzer et al., “Changes in S-phase Fraction and Micronucleus Frequency as Prognostic Factors in Radiotherapy of Cervical Carcinoma,” Radiother. Oncol. 36:128-132 (1995).
Bowen et al., “Abbot Cell-Dyn® 4000 Automated Red Blood Cell Analysis Compared with Routine Red Blood Cell Morphology by Smear Review,” Laboratory Hematology 4:45-57 (1998).
Supplemental European Search Report from corresponding EP 04777169.6.
Choy et al., “Density-Gradient Enrichment of Newly-Formed Mouse Erythrocytes: Application to the Micronucleus Test, Mutation Research,” 130:159-164 (1984).
Riley et al., “Reticulocytes and Reticulocyte Enumeration,” J. Clin. Lab. Anal. 15:267-294 (2001).
Weiss, D.J., “Application of Flow Cytometric Techniques to Veterinary Clinical Hematology,” Vet. Clin. Pathol. 31 (2):72-82 (2002).
Abe et al., “Micronuclei in Human Bone Marrow Cells: Evaluation of the Micronucleus Test Using Human Leukemia Patients Treated with Antileukemic Agents,” Mutat. Res. 130:113-120 (1984).
Abramsson-Zetterberg et al., “Human Cytogenetic Biomonitoring Using Flow-Cytometric Analysis of Micronuclei in Transferrin-Positive Immature Peripheral Blood Reticulocytes,” Environ. Mol. Mutagen. 36:22-31 (2000).
Abramsson-Zetterberg et al., “The Micronucleus Test in Rat Erythrocytes From Bone Marrow, Spleen and Peripheral Blood: The Response to Low Doses of Ionizing Radiation, Cyclophosphamide and Vincristine Determined by Flow Cytometry,” Mutat. Res. 423:113-124 (1999).
Anwar et al., “Chromosomal Aberrations and Micronucleus Frequency in Nurses Occupationally Exposed to Cytotoxic Drugs,” Mutagenesis 9:315-317 (1994).
Asanami et al., “The Suitability of Rat Peripheral Blood in Subchronic Studies for the Micronucleus Assay,” Mutat. Res. 347:73-78 (1995).
Berg-Drewniok et al., “Increased Spontaneous Formation of Micronuclei in Cultured Fibroblasts of First-degree Relatives of Familial Melanoma Patients,” Cancer Genet. Cytogenet. 97:106-110 (1997).
Bhattathiri et al., “Serial Cytological Assay of Micronucleus Induction: A New Tool to Predict Human Cancer Radiosensitivity,” Radiother. Oncol. 41:139-142 (1996).
Burrill et al., “Heritability of Chromosome Radiosensitivity in Breast Cancer Patients: A Pilot Study with the Lymphocyte Micronucleus Assay,” Int. J. Radiat. Biol. 76(12):1617-1619 (2000).
CAS Registry No. 25535-16-4 (Last Updated Dec. 2000).
CAS Registry No. 67-56-1 (Last Updated May 2006).
CAS Registry No. 9001-99-4 (Last Updated Dec. 2004).
CAS Registry No. 9041-08-1 (Last Updated May 2006).
Choy & MacGregor, “Density-Gradient Enrichment of Newly-Formed Mouse Erythrocytes: Application to the Micronucleus Test,” Mutat. Res. 130:159-164 (1984).
Dertinger et al., “Enumeration of Micronucleated CD71-positive Human Reticulocytes with a Single-laser Flow Cytometer,” Mutat. Res. 515:3-14 (2002).
Dertinger et al., “Malaria-Infected Erythrocytes Serve as Biological Standards to Ensure Reliable and Consistent Scoring of Micronucleated Erythrocytes by Flow Cytometry,” Mutat. Res. 464:195-200 (2000).
Dertinger et al., “Micronucleated CD71-postitive Reticulocytes: A Blood-Based Endpoint of Cytogenetic Damage in Humans,” Mutat. Res. 542:77-87 (2003).
Dertinger et al., “Three-Color Labeling Method for Flow Cytometric Measurement of Cytogenetic Damage in Rodent and Human Blood,” Environ. Mol. Mutagen. 44:427-435 (2004).
Doneda et al., “High Spontaneous Chromosome Damage in Lymphocytes From Patients With Hereditary Megaduodenum,” Mutat. Res. 348:33-36 (1995).
Fenech, “Biomarkers of Genetic Damage for Cancer Epidemiology,” Toxicology 181-182:411-416 (2002).
Fenech, “The Cytokinesis-Block Micronucleus Assay in Nucleated Cells,” Mutat. Environ. Part B:195-206 (1990).
Fenech, “The Cytokinesis-Block Micronucleus Technique: A Detailed Description of the Method and its Application to Genotoxicity Studies in Human Populations,” Mutat. Res. 285:35-44 (1993).
Goetz et al., “Relationship Between Experimental Results in Mammals and Man. I. Cytogenetic Analysis of Bone Marrow Injury Induced by a Single Dose of Cyclophosphamide,” Mutat. Res. 31:247-254 (1975).
Grawé et al., “Evaluation of the Reticulocyte Micronucleus Assay in Patients Treated with Radioiodine for Thyroid Cancer,” Mutat. Res. 583:12-25 (2005).
Guo et al., “A Significant Correlation Between Clonogenic Radiosensitivity and the Simultaneous Assessment of Micronucleus and Apoptotic Cell Frequencies,” Int. J. Radiation Biol. 75:857-864 (1999).
Hamada et al., “Evaluation of the Rodent Micronucleus Assay by a 28-Day Treatment Protocol: Summary of the 13th Collaborative Study by the Collaborative Study Group for the Micronucleus Test (CSGMT)/Environmental Mutagen Society of Japan (JEMS)-Mammalian Mutagenicity Study Group (MMS),” Environ. Mol. Mutagen. 37:93-110 (2001).
Hayashi et al., “An Application of Acridine Orange Fluorescent Staining to the Micronucleus Test,” Mutat. Res. 120:241-247 (1983).
Hayashi et al., “In Vivo Rodent Erythrocyte Micronucleus Assay. II. Some Aspects of Protocol Design Including Repeated Treatments, Integration With Toxicity Testing, and Automated Scoring,” Environ. Mol. Mutagen. 35
Gabel Gailene R
Litron Laboratories, Ltd.
Nixon & Peabody LLP
LandOfFree
Method for enumeration of mammalian micronucleated... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for enumeration of mammalian micronucleated..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for enumeration of mammalian micronucleated... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2693900