Method for enrichment of natural antisense messenger RNA

Details Method for enrichment of natural antisense messenger RNA Method for enrichment of natural antisense messenger RNA

2006-01-17

2006-01-17

LeGuyader, John L. (Department: 1635)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C435S091210, C435S091510, C536S023100, C536S024100, C536S024500

Reexamination Certificate

active

06986988

ABSTRACT:
A method for enrichment of natural antisense mRNA which involves hybridization of cDNA obtained from sense RNA with cDNA obtained from antisense RNA, followed by DNA polymerase treatment of the sense-antisense hybrid DNA molecule. A natural antisense library can be generated by cloning of sense-antisense hybrid DNA molecules in a vector.

REFERENCES:
patent: 5891636 (1999-04-01), Van Gelder et al.
patent: WO 99/27113 (1988-06-01), None
patent: WO 99/60164 (1999-11-01), None
Vanhee-Brossollet et al, “Do natural antisense transcripts make sense in eukaryotes?”,Genie211:1-9 (1998).
XP-002171236 -Dolnick, “Cloning and characterization of a naturally occurring antisense RNA to human thymidylate synthase mRNA”,Nucleic Acids Research, 21(8) 1747-1752 (1993).

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