Method for enriching short-chain nucleic acids

Details Method for enriching short-chain nucleic acids Method for enriching short-chain nucleic acids

2011-07-12

2011-07-12

Gakh, Yelena G (Department: 1777)

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-o

C436S063000, C436S161000, C436S178000

Reexamination Certificate

active

07977109

ABSTRACT:
The present invention relates to a method for enriching nucleic acids with a length of not more than 300 nucleotides. The invention also relates to a kit for enriching nucleic acids with a length of not more than 300 nucleotides, to the use of such a kit, to the use of an anion exchange matrix and to a method for treating a disease.

REFERENCES:
patent: 5523231 (1996-06-01), Reeve
patent: 5990301 (1999-11-01), Colpan et al.
patent: 6037465 (2000-03-01), Hillebrand et al.
patent: 6284470 (2001-09-01), Bitner et al.
patent: 6410274 (2002-06-01), Bhikhabhai
patent: 2005/0079535 (2005-04-01), Kirchgesser et al.
patent: 2007/0172855 (2007-07-01), Bitner et al.
patent: 2010/0256351 (2010-10-01), Chen et al.
patent: WO 83/03920 (1983-11-01), None
patent: WO 03/080834 (2003-10-01), None
McNeff, Clatyton et al. “High-performance anion-exchange chromatography of oligonucleotides and oligodeoxynucleotides on quaternized polyethylenimine-coated zirconia.” Analytical Chemistry (1995) 67 p. 2350-2353.
Eon-Duval, Alex et al. “Precipitation of RNA impurities with high salt in a plasmid DNA purification process: use of experimental design to determine reaction conditions.” Biotechnology and Bioengineering (2003) 83 544-553.
Yang, Yu et al. “High performance DNA purification using a novel ion exchange matrix.” Journal of Biomolecular Techniques (2008) 19 205-210.
International Search Report for PCT/EP2006/069484, dated Apr. 19, 2007, English and German Form PCT/ISA1210—(7 pages).
Guenther, R. H. et al., “Purification of transfer RNA species by single-step ion-exchange high-performance liquid chromatorgraphy”; Journal of Chromatography, pp. 79-87, vol. 444, Jul. 1, 1988, XP0092447076, ISSN: 0021-9673.
Drager, R. R. et al., “High-performance anion-exchange chromatography of oligonucleotides”; Analytical Biochemistry, 1985, United States, pp. 47-56, vol. 145, No. 1, 1985, XP009080610.
Lawson, T. G. et al., “Separation of synthetic oligonucleotides on columns of microparticulate silica coated with crosslinked polyethylene imine”, Analytical Biochemistry, pp. 85-93, vol. 133, No. 1, Aug. 1983, XP009080633, ISSN: 0003-2697.
Chaing, C. L. et al., “Application of superparamagnetic nanoparticles in purification of plasmid DNA from bacterial cells”; Journal of Chromatography B; Biomedical Sciences & Applications, pp. 54-60, vol. 822, No. 1-2, Aug. 5, 2005, Elsevier, Amsterdam, NL, XP0049873011, USSN: 1570-0232.
Sambrook, J. et al., “Isolation of High-molecular-weight DNA from Mammalian Cells”, pp. 9.14-9.19, Analysis and Cloning of Eukaryotic Genomic DNA, Molecular Cloning, A Laboratory Manual, second edition, 1989, Cold Spring Harbor Laboratory Press.

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