Method for enriching adherent monocyte populations

Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...

Reexamination Certificate

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C424S534000

Reexamination Certificate

active

07393628

ABSTRACT:
The present invention provides methods and apparatus for the rapid and efficient collection and purification of activated monocytes. The methods and apparatus of the present invention provide means for effecting the collection and purification in an aseptic environment. The method involves filtering a blood component mixture through a monocyte-adhering filter; washing the blood component mixture; backflushing the filter with a physiological solution; and backflushing the filter with Dextran-40/serum albumin.

REFERENCES:
patent: 5665233 (1997-09-01), Fukuda et al.
patent: 6120474 (2000-09-01), Okuda et al.
patent: 6129853 (2000-10-01), Sasayama et al.
patent: 7-80062 (1995-03-01), None
Ebner et al., “Generation of large numbers of human dendritic cells from whole blood passaged through leukocyte removal filters: an alternative to standard buffy coats”, J. Immunological Methods 252 : 93-104 (2001).

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