Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-08-01
1998-10-20
McKelvey, Terry A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 701, 435 691, C12P 2106
Patent
active
058245111
ABSTRACT:
A method for increasing the production of desired hemoproteins is described in which the rate-limiting step in a heme biosynthetic pathway is deregulated to increase the quantity of the rate-limiting enzyme. In yeast, transforming cells with the HEM2 gene increases the quantity of the rate-limiting enzyme ALA dehydratase. Free heme concentrations are reduced in cells by providing heme binding agents that complexes with overproduced heme, thereby reducing or eliminating heme feedback inhibition in the cell. The present invention permits investigation of other factors effecting the heme biosynthetic pathway by reducing interference from heme feedback inhibition within that pathway.
REFERENCES:
patent: 4849348 (1989-07-01), Marcker et al.
patent: 4902620 (1990-02-01), Bard et al.
patent: 5049493 (1991-09-01), Khosla et al.
patent: 5242892 (1993-09-01), Rebeiz
patent: 5635375 (1997-06-01), Kraus et al.
Adachi et al., Oxygen Binding and Other Physical Properties of Human Hemoglobin Made in Yeast, Protein Engineering, vol. 5, No. 8, pp. 807-810, 1992.
Borralho et al., In Situ Assay for 5-Aminolevulinate Dehydratase and Application to the Study of a Carbolite Repression-Resistant Sacchromyces cerevisiae Mutant Journal of Bacteriology, Oct., 1983, pp. 141-147, vol. 156, No. 1.
Borralho et al., "Parallel Changes in Catabolite Repression of Haem Biosynthesis and Cytochromes in Repression-resistant Mutants of Saccharomyces cerevisiae", Journal of Gen. Microbiology, vol. 13, pp, 1217-1227, 1989.
Borralho et al. "Purification of .delta.-Aminolevulinate Dehydratase from Genetically Engineered Yeast", Yeast, vol. 6: 319-330 (1990).
Carvajal, "Isolation and Characterization of a New Mutant of Saccharomyces cerevisiae with Altered Synthesis of 5-Aminolevulinic Acid", Journal of Bacteriology, Jun., 1990, pp. 2855-2861, vol. 172, No. 6.
Coghlin et al. "Structural and Functional Characterisation of Recombinant Human Haemoglobin A Expressed in Saccharomyces cerevisiac," European Journal of Biochemistry, vol. 207, pp. 931-993, 1992.
De Llano et al., "Recombinant Human Sickle Hemoglobin Expressed in Yeast", Proc. Natl. Acad. Sci. USA, vol. 90, pp. 918-922, Feb., 1993.
Grandchamp et al., "Enzymes of the Name Biosynthesis Pathway: Recent Addresses in Molecular Genetics", Seminars in Hematology, vol. 25, No. 4, pp. 303-311, Oct., 1988.
Mattoon, et al., "Coordinate Regulation of Mitochondrial Cytochrome Formation in Saccharomyces cerevisiae" Genetics of Respriratory Enzymes in Yeasts (Materials of the International Conference Karpacz in 1990), pp. 15-22.
Mattoon, "Molecular Genetics of Heme and Cytochrome Biosynthesis", Anal. Acad. Nac. Cs. Ex. Fis. Nat., Buenos Aires, tomo. 40, 1988, pp. 87-94.
Meussdorfer et al., Effect of Mitochondrial Cytochromes and Home Content on Cytochrome P450 in Saccharomyces cerevisiae, Journal of General Microbiology vol. 132, pp. 2187-2193, 1986.
Myers et al., "Characterization of the Yeast HEM2 Gene and Transcriptional Regulation of COX5 and COR1 by Heme", The Journal of Biological Chemistry, vol. 262, No. 35, pp. 16822-16828, December Issue, 1987.
Oechsner et al., Expression of Yeast Cytochrome c.sup.1 is Controlled at the Transcriptional Level by Glucose, Oxygen and Haem, Mol. Gen. Genet., 192, pp. 447-459.
Ogden et al., Expression and Assembly of Functional Human Hemoglobin in S. Cerevisiae, Biomat., Art, Cells & Immob. Biotech, 20 (2-4) pp. 473-475 (1992).
Schauer et al., Heterologous Expression of Human 5-Aminolevulinate Dehydratase in Saccharomyces cerevisiae, Current Genetics, vol. 17, pp. 106, 190.
Wagenbach et al., Synthesis of Wild Type and Mutant Hman Hemoglobins in Saccharomyces Cerevisiae, Biotechnology, vol. 9, pp. 57-61, Jan., 1991.
Bajszar George
Mattoon James R.
McKelvey Terry A.
University Technology Corporation
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