Method for enhancing staining of microorganisms

Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or...

Reexamination Certificate

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Reexamination Certificate

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11172057

ABSTRACT:
The invention is directed to a method and composition of matter for enhancing the staining of tissue or features of interest therein by applying an effective amount of a bile salt in combination with a special stain to a tissue suspected of harboring a microorganism or other feature of diagnostic interest. The particular invention herein is directed to detectingH. pylori, the microorganism responsible for gastrointestinal ulcers. The composition of matter is a sensitizer for Alcian Yellow stain.

REFERENCES:
patent: 5853748 (1998-12-01), New
patent: 2001/0050810 (2001-12-01), Lorincz
patent: WO96/06182 (1996-02-01), None
Kabasawa et al (Japanese Journal of Ophthamology vol. 21, No. 3, pp. 348-354, 1977).
Anim, et al, “Assessment of Different Methods for Staining Helicobacter pylori in Endoscopic Gastric Biopsies,” ACTA Histochem., Urban & Fischer Verlag, vol. 102, pp. 129-137, (2000).
Leung, et al, “Rapid Staining Method for Helicobacter pylori in Gastric Biopsies,” Journal of Histotechnology, vol. 19 (No. 2), pp. 131-132, (1996).
Vartanian, et al, “A Novel Alcian Yellow-Toluidine Blue (Leung) Stain for Helicobacter Species: Comparison with Standard Stains, a Cost-Effectiveness Analysis, and Supplemental Utilities,” Modern Patholoy, vol. 11 (No. 1), pp. 72-78, (1998).
Llewellyn B., “Alcian Yello”, URL <http://members.pgonline.com/˜bryand/StainsFile/dyes/12840.html.

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