Method for enhancing signal release from liposomes using water-m

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals

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436829, 424450, 252700, 435968, G01N 33546, G01N 33543

Patent

active

050932705

ABSTRACT:
A method for releasing encapsulated marker molecules from within liposomes comprises contracting the liposomes with an effective amount of a water-miscible alcohol. The chemiluminescence of acridinium esters is enhanced by oxidizing the acridinium ester in the presence of an effective amount of a water-miscible alcohol.

REFERENCES:
patent: 4708933 (1987-11-01), Huang et al.
patent: 4814270 (1989-03-01), Piran
patent: 4927769 (1990-05-01), Chang et al.
Ellefson et al., "Chapter 10-Lipids and Lipoproteins", Fundamentals of Clinical Chemistry, pp. 474, 489-491, 507-511, 1976.

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