Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1992-03-30
1995-03-28
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 4, 435 29, 435810, 422 52, 422 61, 436172, 436808, C12Q 128, G01N 2176
Patent
active
054016401
ABSTRACT:
A method for enhancing luminol chemiluminescence assays for superoxide anion comprising an orthovanadate salt, such as sodium orthovanadate. Orthovanadate enhances photon emission when luminol is cleaved by superoxide anion. The orthovanadate salt is non-cytotoxic and non-denaturing to intact cells. A method comprises reacting luminol in the presence of an orthovanadate anion with an analyte suspected of containing superoxide anion and measuring the photon output. A kit comprises a predetermined amount of luminol and orthovanadate in separate containers.
REFERENCES:
patent: 4598044 (1986-07-01), Kricka et al.
patent: 4729950 (1988-03-01), Kricka et al.
patent: 4835101 (1989-05-01), Kao et al.
patent: 4842997 (1989-06-01), Carter et al.
patent: 4959182 (1990-09-01), Schaap
patent: 4962192 (1990-10-01), Schaap
US Patent & Trademark Translation of "New Chemiluminescent Reaction of Luminol in the Presence of Vanadium Salts", Pilipenko et al, Ukr. Khim Zh. (Russ. ed) 39 (1); 73-5, 1973.
Robbins et al; "Basic Pathology"; 4th ed.; 1987; pp. 35-36; W. B. Sanders Company. Phil. Pa.
Liochev, S. I., Fridovich, I. (1989) Arch. Biochem. Biophys. 275, 40-43.
Yoshino, S. Sullivan, S. G. and Stern, A. (1989) Arch. Biochem. Biophys. 272, 76-80.
Coulombe, R. A. Jr., Briskin, D. P., Keller, R. J., Thornley, W. R. and Sharma, R. P. (1987) Arch. Biochem. Biophys. 255, 267-273.
Reif, D. W., Coulombe, R. A. Jr., and Aust, S. D. (1989) Arch. Biochem. Biophys. 270, 137-143.
Rau, M., Patole, M. S., Jihaya, S., Ramakrishna Kurup, C. K., and Ramasarma, T. (1987) Mol. Cell Biochem. 75, 151-159.
Liochev, S., Ivancheva, E., and Fridovich, I. (1989) Arch. Biochem. Biophys. 269, 188-193.
Tran Hien
Trustees of Dartmouth College
Warden Robert J.
LandOfFree
Method for enhancing detection of superoxide anion does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for enhancing detection of superoxide anion, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for enhancing detection of superoxide anion will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2249870