Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2005-09-20
2005-09-20
Marx, Irene (Department: 1651)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S171000, C435S244000, C435S155000
Reexamination Certificate
active
06946277
ABSTRACT:
This invention provides a method for enhancing the cellobiase activity of the strainTermitimyces clypeatususing 2-deoxy-D-glucose as glycosylation inhibitor, said process comprises inoculating and growing mycelial culture of (the edible mushroom)Termitimyces clypeatus, in sterilized medium containing 0.05 to 5.0% of 2-deoxy-D-glucose in addition to 0.5 to 2.0% of cellobiose, succinate—0.5%, 2 to 3% of ammonium di hydrogen phosphate and conventional micro-nutrients at pH between 3 to 8 and incubating at temperatures between 20-35° C. under shaking in aerobic conditions, and separating the culture medium by known methods, and using the culture filtrate directly as the source of the enzyme cellobiase and also for endo-glucanase and cellobiohydrolase for use in cellulose hydrolysis.
REFERENCES:
Kubicek et al., Canadian Journal of Microbiology, vol. 33, pp. 698-703.
Bhattacharyya et al., “Purification and characterization of an extracellular beta-xylosidase fromTermitomyces clypeatus”, Biotechnology Progross 13 (6): 822-825 (1997).
Roy et al., “Development of high molar mass cellobiase comples by spontaeous protein-protein interaction in the culture filtrate ofTermitomyces clypeatus”, Folia Microbiologica 39 (6): 463-70 (1994).
Sengupta et al., “Purification and Characterization of a beta glucosidase cellobiase fromTermitomyces clypeatus”, Biochim. Biophys. Acta 1076 (2): 215-20 (1991).
Khowala Suman
Mukherjee Sumana
Council of Scientific and Industrial Research
Harness Dickey & Pierce PLC
Marx Irene
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