Method for enhancing amplification in the polymerase chain react

Details Method for enhancing amplification in the polymerase chain react Method for enhancing amplification in the polymerase chain react

1995-06-06

1997-08-12

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 912, 435 5, 435 6, 536 243, 536 2431, 536 2432, 536 2433, C12P 1934, C12Q 170, C12Q 168, C07H 2104

Patent

active

056564619

ABSTRACT:
A process for producing a particular nucleic acid sequence from a given sequence of DNA or RNA in amounts which are large compared to the amount initially present, using PNAs in conjunction with the polymerase chain reaction (PCR) is disclosed. The process may be used to amplify relatively long DNA or RNA sequences of about 600 base pairs or longer, while reducing the generation of anomalous products and the tendency to preferentially amplify smaller nucleic acid (DNA or RNA) fragments when present in the heterozygous state with a larger allele.

REFERENCES:
patent: 5075217 (1991-12-01), Weber
patent: 5436149 (1995-07-01), Barnes
patent: 5512462 (1996-04-01), Cheng

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