Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide contains a tissue – organ – or cell...
Reexamination Certificate
2005-02-08
2005-02-08
Kubelik, Anne (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide contains a tissue, organ, or cell...
Reexamination Certificate
active
06852908
ABSTRACT:
The present invention relates to methods for reducing pollen production in plants. In particular, the present invention relates to a method of reducing pollen production in plants, which comprises the steps of: a) introducing into the nucleus of a plant cell a gene construct essentially consisting of a developmentally regulated promoter driving expression of a sequence encoding a mitochondrial transit peptide fused upstream of and in frame with an edited form of the orf224 gene ofBrassica napusmitochondria, wherein said promoter is expressed in mid- to late-stage stamen development; b) selecting for plant cells that have acquired the gene construct in step a); and c) inducing regeneration of selected plant cells to produce a mature plant with reduced pollen production.
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(Ogilvy Renault)
Bérubé Luc
Kubelik Anne
McGill University
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