Method for encapsulating biologically active material including

Stock material or miscellaneous articles – Coated or structually defined flake – particle – cell – strand,... – Particulate matter

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264 41, 264 432, 264 433, 435178, 435182, 43524022, C12N 1104, C12N 1110, B01J 1314, B01J 1302

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active

050843508

ABSTRACT:
Method for encapsulating biologically active material within a semipermeable capsule wherein gelled beads are obtained by suspending the material in a solution of a water-soluble substance which can be reversibly gelled, forming the solution into droplets and gelling the droplets to produce discrete shaped-retaining temporary gelled beads. The improvement comprises the subsequent steps of (a) suspending the gelled beads in a solution of a water-soluble substance which can be reversibly gelled (b) forming droplets from the suspension of (a), thereby obtaining droplets which contain one or more gelled beads of step (a); (c) gelling the droplets of step (b) to produce discrete shaped-retaining temporary gelled beads which contain the gelled beads of step (a); (d) forming semi-permeable membranes on each of the gelled beads of step (c), thereby preventing the incorporation of any biologically active material of step (a) into the membranes and (e) reliquifying the gel within the membranes, thereby dispersing the content of the gelled beads of step (a) within the capsule.

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