Method for eliminating hemolysis interference in an amylase anal

Details Method for eliminating hemolysis interference in an amylase anal Method for eliminating hemolysis interference in an amylase anal

1995-11-20

1998-06-16

Wilson, James O.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435210, C12Q 140

Patent

active

057668728

ABSTRACT:
A method for increasing the accuracy of photometric-based assays for .alpha.-amylase by subjecting a sample to a secondary interrogating beam of radiation at a wavelength distinguishable from a primary interrogating beam of radiation. The secondary interrogating beam of radiation is indicative of an interfering reaction occurring in the absence of analyte at the primary wavelength. The secondary wavelength is outside the absorption spectrum of the analyte of interest. This secondary radiation beam's absorption is proportional to the interfering reaction at the primary wavelength.

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patent: 4233403 (1980-11-01), Menson et al.
patent: 4698300 (1987-10-01), Henkel et al.
patent: 4963479 (1990-10-01), Chavez et al.
Jansen & Wydeveld, Alpha-(p-Nitrophenyl)Maltoside as a Substrate for the Assay of Amylase, Nature, 182, 525-526, Aug. 23, 1958.

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