Method for eliciting an immune response using a gene expression

Details Method for eliciting an immune response using a gene expression Method for eliciting an immune response using a gene expression

1997-10-03

1998-12-08

LeGuyader, John L.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

435 6, 435 691, 4351721, 4351723, 4353201, 435325, 435354, 435356, 435366, A61K 4100, C12Q 168, C12N 1585

Patent

active

058469494

ABSTRACT:
The present invention relates to a gene expression system in eukaryotic cells. In particular, it relates to a self-initiating and self-sustaining gene expression system utilizing an RNA polymerase prebound to a DNA construct before the introduction of the complex into cells. This system is capable of functioning in the cell cytoplasm without integration into the genome of host cells, and does not require host cell factors for initiation and maintenance of gene expression. Therefore, the invention has a wide range of applications in in vitro and in vivo gene expression, including gene therapy in resting cells.

REFERENCES:
patent: 5126251 (1992-06-01), Moss et al.
patent: 5580859 (1996-12-01), Felgner et al.
patent: 5589466 (1996-12-01), Felgner et al.
Deng et al., "High-Efficiency Protein Synthesis form T7 Rna Polymyerase in 3T3 Fibroblasts", Gene 109:193-201 (1991).
Dubendorff and Studier, "Creation of a T7 Autogene: Cloning and Expression of the Gene for Bacteriophage T7 RNA Polymerase under Control of its Cognate Promoter", J Mol Biol 219:61-68 (1991).
Fuerst et al., 1989, "Structure and Stability of mRNA Synthesized by Vaccinia Virus-Encoded Bacteriophage T7 RNA Polymerase in Mammalian Cells," J. Mol. Biol. 206:333-348.
Gao et al., 1993, "Cytoplasmic Gene Expression By Co-Delivery of T7 RNA Polymerase and T7 Promoter Sequence By Cationic Liposme," Cell Biochem. Suppl. (17 Part E), Abstract #206, S307.

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