Method for electroporation

Details Method for electroporation Method for electroporation

1990-08-22

1992-06-23

Doll, John

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435173, 935 52, C12N 1587, C12N 1300

Patent

active

051242591

ABSTRACT:
Disclosed is a method for electroporation with which higher transformation efficiency may be attained than that attained by the conventional methods. In the method of electroporation, cells and DNAs are suspended in a buffer containing potassium ion as a cation and an amino acid ion and/or an organic acid ion as an anion, and substantially not containing chloride ion, and high voltage is applied thereacross.

REFERENCES:
Zimmermann, et al., Effects of External Electric Fields on Cell Membranes Bioelectrochem & Bioenergetics vol. 3, pp. 58-83 1976.
Zhelev, et al. Correlation Between Physical Parameters in Electrofusion and Electroporation of Protoplasts Bioelectrochem & Bioenergetics vol. 20, pp. 155-167 1988.
Zerbib, et al. Electric Field Mediated Transformation: Isolation and Characterization of a TK.sup.+ Subclonk Biochem Biophys Res Comm. vol. 129, No. 3, pp. 611-618 1985.

Affiliated with

Also associated with

Rating

Method for electroporation does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for electroporation, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for electroporation will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-932526