Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Reexamination Certificate
2007-02-13
2007-02-13
Noguerola, Alex (Department: 1753)
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
C204S600000
Reexamination Certificate
active
10297214
ABSTRACT:
The one and two-dimensional separation of proteins extending through the membrane (membrane proteins) is a prerequisite for a complete analysis of the proteome of a functionally intact cell organelle. A substantial drawback of prior art methods for separating is that their applicability is limited to peripheral water-soluble proteins. The aim of the novel method is to electrophoretically separate membrane proteins in one or two dimensions. To this end, membranes from cell organelles, whose membrane protein stock should be separated, are placed on planar supports whose surface was modified using suitable methods in such a manner that the membrane proteins maintain their ability to laterally diffuse in the membrane plane after being placed on the support. By subsequently applying electrical fields, the proteins can be electrophoretically displaced in the membrane plane and, as a result, can be separated in one or two dimensions according to their different charge characteristics.
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patent: 2001/0025791 (2001-10-01), Landau et al.
patent: WO-91/04326 (1991-04-01), None
Xu Chong et al “Two-dimensional electrophoretic profile of human sperm membrane proteins.” vol. 15 No. 6, 1994, pp. 595-602.
Stelzle M et al “Two-dimensional microelectrophoresis I supported lipid bilayers.” vol. 63, No. 5, 1992, pp. 1346-1354.
Bayerl Thomas
Sackmann Erich
Antonelli, Terry Stout and Kraus, LLP.
Noguerola Alex
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