Method for electronic detection of a binding reaction

Details Method for electronic detection of a binding reaction Method for electronic detection of a binding reaction

1986-03-25

1988-12-27

Warden, Robert J.

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

436518, 436531, 436806, 436807, 436809, 204403, G01N 3353

Patent

active

047940891

ABSTRACT:
A method is disclosed for detecting the occurrence of a binding or complex-forming reaction between specific substances by utilizing the binding reaction to complete an electrical circuit, and then measuring a change in the electrical state of this circuit. In a preferred embodiment, a layer of antigen is coated onto a non-conductive base between a pair of electrically conductive layers superposed on the base. Antibodies which react with the foregoing antigen are treated so that they become bound to fine electrically conductive, metallic particles. The electrically conductive particles having antibody bound thereto are then added to the antigen layer deposited on the base and allowed to react therewith. Electrically conductive particles are thereby bound to the base due to the binding reaction between the antigen and antibody to thereby form aggregates of electrically conductive particles which bridge the electrically conductive layers and complete the circuit. Such a method is highly useful for the detection of antigens in the blood serum of a human patient.

REFERENCES:
patent: 3074853 (1963-01-01), Brewer
patent: 3853467 (1974-12-01), Giaever
patent: 3926564 (1975-12-01), Giaever
patent: 4054646 (1977-10-01), Giaver
patent: 4072576 (1978-02-01), Arwin et al.
patent: 4157323 (1979-06-01), Yen et al.
patent: 4180771 (1979-12-01), Guckel
patent: 4191739 (1980-03-01), Hzgiris
patent: 4198389 (1980-04-01), Wadsworth
patent: 4206094 (1980-06-01), Yen et al.
patent: 4219335 (1980-08-01), Ebersole
patent: 4219411 (1980-08-01), Yen et al.
patent: 4225410 (1980-09-01), Pace
patent: 4233144 (1980-11-01), Pace
patent: 4238757 (1980-12-01), Schenck
patent: 4287300 (1981-09-01), Gibbons
patent: 4327073 (1982-04-01), Huang
patent: 4334880 (1982-06-01), Malmros
patent: 4399099 (1983-08-01), Buckles
patent: 4420558 (1983-12-01), De Mey et al.
patent: 4444892 (1984-04-01), Malmros
patent: 4446238 (1984-05-01), De Mey et al.
patent: 4459360 (1984-07-01), Marinkovich
The Journal of Histochemistry and Cytochemistry, A Quantitative Immunoperoxidase Procedure Employing Energy Dispersive X-Ray Analysis.
Derwent Abstract C85-033856.
Patents Abstracts of Japan-Unexamined Applications Sect. P, Section No. 367; vol. 9, No. 151, PO 136.
Bogomolov: Chemical Abstracts 74:71034c (1971).
Bogomolov: Chemical Abstracts 81:20366z (1974).
Vogelzang: Chemical Abstracts 102:28400d (1985).

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