Method for electroblotting

Details Method for electroblotting Method for electroblotting

1984-11-14

1986-05-20

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, G01N 2728

Patent

active

045899657

ABSTRACT:
A rapid and effective method for electroblotting is provided whereby an electrophoretically resolved material in a gelatin sheet is quickly and efficiently transferred to a membrane with high pattern definition and resolution.

REFERENCES:
Bittner, M. et al., "Electrophoretic Transfer of Proteins and Nucleic Acids from Slab Gels to Diazobenzyloxymethyl Cellulose or Nitrocellulose Sheets", Analytical Biochemistry vol. 102, pp. 459-471, (1980).
Gibson, W., "Protease-Facilitated Transfer of High-Molecular-Weight Proteins During Electrotransfer to Nitrocellulose", Analytical Biochemistry, vol. 118, pp. 1-3, (1981).
Towbin, H. et al., "Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications", Proc. of National Acadamy of Sciences, USA, vol. 76, pp. 4350-4354, (1979).

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